Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L.
Identifieur interne : 000276 ( France/Analysis ); précédent : 000275; suivant : 000277Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L.
Auteurs : M. Dupuis [France] ; M. Péan [France] ; P. Chagvardieff [France]Source :
- Plant Cell, Tissue and Organ Culture [ 0167-6857 ] ; 1990-09-01.
Abstract
Abstract: The effects of factors influencing sunflower protoplast isolation yield, plating efficiency (PE) and the early differentiation into embryoids (embryogenic capacity, EC) have been studied. Only hypocotyl-derived protoplasts divided. The variations of PE and EC in the various treatments did not seem to be linked to the protoplast yields. From statistical analysis of the data, we concluded that, the sunflower genotype, the age and height of seedlings, the part of hypocotyl used, the incubation time (from 6 to 16 hours) in enzymes of explants or of protoplasts alone, influenced PE but large variations were detected for EC. A comparison of the factors effecting EC suggested an origin, inside the hypocotyl, of cells able to give rise, after induction, to embryogenic protoplasts.
Url:
DOI: 10.1007/BF00033634
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001163
- to stream Istex, to step Curation: 001163
- to stream Istex, to step Checkpoint: 001325
- to stream Main, to step Merge: 004057
- to stream Main, to step Curation: 003D14
- to stream Main, to step Exploration: 003D14
- to stream France, to step Extraction: 000276
Links to Exploration step
ISTEX:0213150A4D769E140DB6099223186985757A93B1Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L.</title>
<author><name sortKey="Dupuis, M" sort="Dupuis, M" uniqKey="Dupuis M" first="M." last="Dupuis">M. Dupuis</name>
</author>
<author><name sortKey="Pean, M" sort="Pean, M" uniqKey="Pean M" first="M." last="Péan">M. Péan</name>
</author>
<author><name sortKey="Chagvardieff, P" sort="Chagvardieff, P" uniqKey="Chagvardieff P" first="P." last="Chagvardieff">P. Chagvardieff</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:0213150A4D769E140DB6099223186985757A93B1</idno>
<date when="1990" year="1990">1990</date>
<idno type="doi">10.1007/BF00033634</idno>
<idno type="url">https://api.istex.fr/document/0213150A4D769E140DB6099223186985757A93B1/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001163</idno>
<idno type="wicri:Area/Istex/Curation">001163</idno>
<idno type="wicri:Area/Istex/Checkpoint">001325</idno>
<idno type="wicri:doubleKey">0167-6857:1990:Dupuis M:plant:donor:tissue</idno>
<idno type="wicri:Area/Main/Merge">004057</idno>
<idno type="wicri:Area/Main/Curation">003D14</idno>
<idno type="wicri:Area/Main/Exploration">003D14</idno>
<idno type="wicri:Area/France/Extraction">000276</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L.</title>
<author><name sortKey="Dupuis, M" sort="Dupuis, M" uniqKey="Dupuis M" first="M." last="Dupuis">M. Dupuis</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Association pour la Recherche en Bioénergie Solaire, Cen-Cadarache 13108, Saint-Paul-lez-Durance Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Saint-Paul-lez-Durance</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pean, M" sort="Pean, M" uniqKey="Pean M" first="M." last="Péan">M. Péan</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Association pour la Recherche en Bioénergie Solaire, Cen-Cadarache 13108, Saint-Paul-lez-Durance Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Saint-Paul-lez-Durance</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chagvardieff, P" sort="Chagvardieff, P" uniqKey="Chagvardieff P" first="P." last="Chagvardieff">P. Chagvardieff</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Service de Radioagronomie Départment de Biologie, Cen/Cadarache, Laboratoire d'Amélioration des Plantes, 13108, Saint-Paul-Lez-Durance Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Saint-Paul-Lez-Durance</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Plant Cell, Tissue and Organ Culture</title>
<title level="j" type="sub">An International Journal on Biotechnology of Higher Plants</title>
<title level="j" type="abbrev">Plant Cell Tiss Organ Cult</title>
<idno type="ISSN">0167-6857</idno>
<idno type="eISSN">1573-5044</idno>
<imprint><publisher>Kluwer Academic Publishers</publisher>
<pubPlace>Dordrecht</pubPlace>
<date type="published" when="1990-09-01">1990-09-01</date>
<biblScope unit="volume">22</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="183">183</biblScope>
<biblScope unit="page" to="189">189</biblScope>
</imprint>
<idno type="ISSN">0167-6857</idno>
</series>
<idno type="istex">0213150A4D769E140DB6099223186985757A93B1</idno>
<idno type="DOI">10.1007/BF00033634</idno>
<idno type="ArticleID">BF00033634</idno>
<idno type="ArticleID">Art6</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0167-6857</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: The effects of factors influencing sunflower protoplast isolation yield, plating efficiency (PE) and the early differentiation into embryoids (embryogenic capacity, EC) have been studied. Only hypocotyl-derived protoplasts divided. The variations of PE and EC in the various treatments did not seem to be linked to the protoplast yields. From statistical analysis of the data, we concluded that, the sunflower genotype, the age and height of seedlings, the part of hypocotyl used, the incubation time (from 6 to 16 hours) in enzymes of explants or of protoplasts alone, influenced PE but large variations were detected for EC. A comparison of the factors effecting EC suggested an origin, inside the hypocotyl, of cells able to give rise, after induction, to embryogenic protoplasts.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Provence-Alpes-Côte d'Azur</li>
</region>
<settlement><li>Saint-Paul-Lez-Durance</li>
<li>Saint-Paul-lez-Durance</li>
</settlement>
</list>
<tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Dupuis, M" sort="Dupuis, M" uniqKey="Dupuis M" first="M." last="Dupuis">M. Dupuis</name>
</region>
<name sortKey="Chagvardieff, P" sort="Chagvardieff, P" uniqKey="Chagvardieff P" first="P." last="Chagvardieff">P. Chagvardieff</name>
<name sortKey="Pean, M" sort="Pean, M" uniqKey="Pean M" first="M." last="Péan">M. Péan</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000276 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000276 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= France |étape= Analysis |type= RBID |clé= ISTEX:0213150A4D769E140DB6099223186985757A93B1 |texte= Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L. }}
This area was generated with Dilib version V0.6.25. |